GCEBiologyCell Structure and Organisation2022

During the preparation of a temporary slide of an onion epidermal cell, a Form Five student added a drop of iodine solution. The most likely observation is that the

Acell wall stains blue-black and the cytoplasm remains unstained
Bnucleus stains yellow-brown and the vacuole stains blue-black
Ccell wall stains yellow-brown and the nucleus appears more distinctCORRECT
Dchloroplasts stain blue-black indicating the presence of starch
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Why the answer is C, and why the others tempt you.
Iodine solution (Lugol's iodine) stains cellulose cell walls yellow-brown and also stains the nucleus, making cellular structures more visible and distinct under the microscope. Onion epidermal cells do not contain chloroplasts and their vacuoles contain cell sap rather than starch, so a blue-black reaction indicating starch would not be expected in the vacuole. The iodine enhances contrast, making the nucleus appear more clearly defined.
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